BHMT Antikörper (AA 13-386)

Produktnummer ABIN7599940

Dieser Anti-BHMT Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von BHMT in WB, IHC, ELISA, FACS, IF und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für BHMT Antikörper (AA 13-386) (ABIN7599940)

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BHMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-BHMT Antikörper

Bindungsspezifität: AA 13-386

Verwendungszweck: Anti-BHMT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BHMT Antibody Picoband® (ABIN7599940). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BHMT recombinant protein (Position: I13-K386).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Garrow, T. A. Purification, kinetic properties, and cDNA cloning of mammalian betaine-homocysteine methyltransferase. J. Biol. Chem. 271: 22831-22838, 1996. 2. Li, F., Feng, Q., Lee, C., Wang, S., Pelleymounter, L. L., Moon, I., Eckloff, B. W., Wieben, E. D., Schaid, D. J., Yee, V., Weinshilboum, R. M. Human betaine-homocysteine methyltransferase (BHMT) and BHMT2: common gene sequence variation and functional characterization. Molec. Genet. Metab. 94: 326-335, 2008. 3. Park, E. I., Garrow, T. A. Interaction between dietary methionine and methyl donor intake on rat liver betaine-homocysteine methyltransferase gene expression and organization of the human gene. J. Biol. Chem. 274: 7816-7824, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Andere Bezeichnung: BHMT

Hintergrund:

Synonyms: Neurocan core protein, Chondroitin sulfate proteoglycan 3, NCAN, CSPG3, NEUR,

Tissue Specificity: Brain.

Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.

Molekulargewicht: 50 kDa

Gen-ID: 635

UniProt: Q93088

Pathways: Methionine Biosynthetic Process